Title

Isolation And Characterization Of Twelve Microsatellite Loci For Rockfish (sebastes)

Document Type

Article

Publication Date

5-1-1999

Publication Title

Marine Biotechnology

Department

Biology

Abstract

We describe the first microsatellites for rockfishes in the diverse genus Sebastes. Clones containing microsatellites were isolated from the genomic library of a quillback rockfish, Sebastes maliger. Twelve microsatellites are characterized; six of these are polymorphic in quillback rockfish, and eight are polymorphic in at least one rockfish species on which they were tested. The number of alleles per variable locus ranged from 4 to 15 and averaged 6.8. The expected heterozygosities ranged from 0.38 to 0.79 and averaged 0.60 in these loci. These loci should prove valuable in studies examining species identification, population genetics, hybridization, paternity, kinship, and microsatellite evolution.

Volume

1

Issue

3

pp.

311-315

ISSN

1436-2228