Title

An electrically-stimulate optically-record microsystem based on active CMOS multi-electrode array for dissociated cell cultures

Document Type

Presentation or Lecture

Publication Date

4-21-2011

Publication Title

IEEE/NIH

Conference or Event

Life Science Systems and Applications Workshop (LiSSA)

Department

Neuroscience

Abstract

Calcium fluorescense-based optical recording combines with patch-clamp stimulation has become the standard technique for analyzing neural network behavior. At best, stimulation is limited to only a few channels in this case. Passive multi-electrode per mm2. Here, we report an active multi-electrode array, constructed with a standard complementary metal-oxide-semiconductor (CMOS) technology, to perform localized extracellular stimulation of dispersed cell cultures. A 256x256 array integrated with in-pixel stimulators on a 4-by-4 mm2 CMOS chip noninvasively stimulate hippocampal cells cultured on chip at cellular resolution. Combined with calcium imaging using high-affinity indicators, we demonstrate the ability to observe spatiotemporal dynamics of neural activity.