Title

Effect of high water temperature on apoptosis in Zebrafish (Danio rerio) ovarian tissue

Faculty Advisor

DeMarais, Alyce

Area of Study

Science and Mathematics

Publication Date

Summer 2015

Abstract

Elevated water temperature has significant effects on the biology of fishes, which raises major concerns about the impacts of climate change on the health of fish populations. High water temperature has been shown to induce apoptosis, or programmed cell death, in various tissues of fish. However, the effects of high water temperature on apoptosis in the ovaries of fish has been little-studied, despite its implications for the overall reproductive health of fishes. This study addresses the effects of high water temperature on apoptosis in the ovarian tissue of zebrafish. qPCR was used to quantify the expression of genes related to apoptosis in fish exposed to a control water temperature (23°C) and a high temperature (32°C) for two weeks. Initial results suggest that apoptosis may actually have been reduced in the high water temperature treatment, though the results were variable and more replications are needed.

Publisher

University of Puget Sound

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