Title
Dissecting the Functional Domains of a Nonenveloped Virus Membrane Penetration Peptide
Document Type
Article
Publication Date
7-2009
Publication Title
Journal of Virology
Department
Chemistry
Abstract
Recent studies have established that several nonenveloped viruses utilize virus-encoded lytic peptides for host membrane disruption. We investigated this mechanism with the “gamma” peptide of the insect virus Flock House virus (FHV). We demonstrate that the C terminus of gamma is essential for membrane disruption in vitro and the rescue of immature virus infectivity in vivo, and the amphipathic N terminus of gamma alone is not sufficient. We also show that deletion of the C-terminal domain disrupts icosahedral ordering of the amphipathic helices of gamma in the virus. Our results have broad implications for understanding membrane lysis during nonenveloped virus entry.
ISSN
0022-538X
Provider Link
Citation
Banerjee, M., Khayat, R., Walukiewicz, H.E., Odegard, A.L., Schneemann, A., Johnson, J.E. 2009. Dissecting the Functional Domains of a Nonenveloped Virus Membrane Penetration Peptide. Journal of Virology 83 (13): 6929-33.