An electrically-stimulate optically-record microsystem based on active CMOS multi-electrode array for dissociated cell cultures

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Presentation or Lecture

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Conference or Event

Life Science Systems and Applications Workshop (LiSSA)




Calcium fluorescense-based optical recording combines with patch-clamp stimulation has become the standard technique for analyzing neural network behavior. At best, stimulation is limited to only a few channels in this case. Passive multi-electrode per mm2. Here, we report an active multi-electrode array, constructed with a standard complementary metal-oxide-semiconductor (CMOS) technology, to perform localized extracellular stimulation of dispersed cell cultures. A 256x256 array integrated with in-pixel stimulators on a 4-by-4 mm2 CMOS chip noninvasively stimulate hippocampal cells cultured on chip at cellular resolution. Combined with calcium imaging using high-affinity indicators, we demonstrate the ability to observe spatiotemporal dynamics of neural activity.