Faculty Advisor

Grinstead, Jeffrey

Area of Study

Science and Mathematics

Publication Date

Summer 2011


The sequenced genome of the gram-negative predatory bacterium Bdellovibrio bacteriovorus contains a gene that encodes for malA, a putative maltase. Given the bacterium's observed disuse of prey carbohydrates, the gene's presence is mysterious. That characterization of the enzyme and studies of its activity and specificity can be better carried out, it is necessary to obtain pure enzyme. Protein was collected from lysed cultures of Top10/pmalA E. coli. Attempted purification by ion-exchange chromatography with DEAE columns produced significantly purer protein; SP ion exchange columns were unsuccessful, as were heparin and hydroxyapatite affinity columns. Gel filtration chromatography should prove a useful method for further purification.


University of Puget Sound

Trecker_summer_2011_reflection.doc (62 kB)
Reflecting on Summer 2011 and the Fall Student Research Symposium

Trecker_summer_2011_poster.ppt (6216 kB)
Poster in MS Powerpoint format

Included in

Biochemistry Commons