Faculty Advisor

Kim, Jung

Area of Study

Science and Mathematics

Publication Date

Summer 2019


The purpose of this study was to determine the expression of genes in the IGF-1-mediated pathway involved in protein synthesis and degradation on muscle regeneration after cardiotoxin (CTX)-induced injury, which may be causing muscle hypertrophy. Quantitative Real-Time Polymerase Chain Reaction (qPCR) and western blots were used to quantify expression of degradation and synthesis markers respectively. Absolute and relative tibialis anterior (TA) muscle weights were significantly greater than the respective control after 2-, 3-, and 4-weeks CTX. There were no significant differences in gene expression of atrogin-1, MuRF-1, or myostatin between the CTX-injured and the Control TA muscle at the 2-, 3-, and 4-week time points.




University of Puget Sound