mRNA Expression of Muscle Differentiation Markers in Wild Type and MMP-2 Knockout Mice After Functional Overload of the Plantaris Muscle

Authors

Natalie MacIlwaine, University of Puget SoundFollow
Gary McCall, University of Puget Sound
Jung Kim, University of Puget Sound

Faculty Advisor

Jung Kim

Area of Study

Science and Mathematics

Publication Date

Summer 2021

Abstract

This project aims to investigate the role of an enzyme called MMP2 in skeletal muscle hypertrophy after functional overload of the plantaris muscle in wild-type and MMP2 knockout mice. Specifically, we examined the expression of muscle differentiation markers MyoD and myogenin using Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) in the functionally overloaded plantaris muscle from wild type (WT) and MMP-2 knockout (KO) mice. We hypothesized that the decreased hypertrophic response to FO in MMP-2 KO mice is due to impaired ECM remodeling and impairment of protein synthesis pathways.

Award

Sci/Math Summer Research Scholar

Recommended Citation

MacIlwaine, Natalie; McCall, Gary; and Kim, Jung, "mRNA Expression of Muscle Differentiation Markers in Wild Type and MMP-2 Knockout Mice After Functional Overload of the Plantaris Muscle" (2021). Summer Research. 397.
https://soundideas.pugetsound.edu/summer_research/397

Rights

Default Rights Statement.

Publisher

University of Puget Sound