The Role of MMP-2 on Satellite Cells and Hypertrophy of the Mouse Plantaris Muscle After Functional Overload
Area of Study
Science and Mathematics
Skeletal muscle makes up 40-50% of the human body and has the capability to adapt to environmental changes. However, we do not know much about how these adaptations occur at the cellular level. Within the extracellular matrix surrounding each muscle fiber, there are satellite cells and matrix metalloproteinases which have been shown to play a role in muscle regeneration and repair. To understand the mechanisms of these adaptations, this study observed satellite cells in the plantaris muscle of wild type as well as mice with matrix metalloproteinase-2 (MMP-2) taken out. A functional overload model was used and mice were observed 0-days, 2-, 4-, and 8- weeks after this functional overload. Immunohistochemistry was performed to stain the basal lamina of each muscle fiber, the satellite cells, and nuclei. Images using an epifluorescence microscope were taken to quantify satellite cells and the data showed that there was a significant difference in satellite cells between the 0-day and the 2-, 4-, and 8-week wild type and MMP-2 knockout mice. However, the MMP-2 knockout mice had less satellite cells than the wild type mice suggesting that the absence of MMP-2 may lead to a decreased activation in satellite cells.
Santos, Layla, "The Role of MMP-2 on Satellite Cells and Hypertrophy of the Mouse Plantaris Muscle After Functional Overload" (2021). Summer Research. 406.
University of Puget Sound