Quantification of Skeletal Muscle Synthesis Pathway Proteins in Wild Type and MMP-2 Knockout Mice After Functional Overload of the Plantaris Muscle
Area of Study
Science and Mathematics
Skeletal muscle is the most abundant muscle in the human body. It is highly plastic, which allows the muscle to adapt to changing functional needs including exercise and injury. Previous studies have highlighted the role of the extracellular matrix (ECM) in skeletal muscle plasticity. The ECM is primarily composed of proteoglycans and collagens and can regenerate in response to injury, exercise, or disease. This regeneration of the ECM is partially facilitated by matrix metalloproteinases (MMPs), enzymes responsible for degrading extracellular proteins. The disruption of ECM synthesis and MMP activity has been shown to affect skeletal muscle regeneration. The purpose of this study was to examine the effects of MMP-2 knockout (KO) on protein synthesis pathway in a functional overload (FO) model. Western blots were conducted to measure the synthesis pathway protein (IGF-1, Akt, mTOR, and S6K1) concentration in wild type and KO mice plantaris muscle.
Kenney, Naia N. and Kim, Jung, "Quantification of Skeletal Muscle Synthesis Pathway Proteins in Wild Type and MMP-2 Knockout Mice After Functional Overload of the Plantaris Muscle" (2021). Summer Research. 416.
University of Puget Sound